Effect of Sub-Lethal Concentrations of Manganese on Sperm Motility of the Caspian lamprey (Cspimyzon wagneri)

Authors
M. Nasrollah Pourmoghadam
S. Eagdari
B. Mojazi Amiri
H. Poorbagher
N. Nemati Mobin
Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
Abstract
The pollution of aquatic ecosystems may affect natural reproduction of fish populations by decreasing the fertilization efficiency. Among the various sources of pollution, heavy metals are important group, being found in both freshwater and marine environment. The present study investigated changes of sperm characteristics of Caspimyzon wagneri when exposed to Manganese (Mn). Spermatozoids were exposed to 0 (control), 0.01, 0.1, 1, 10, 100 and 1000 mg l‑1 of Mn, duration of sperm motility and percent of motile sperms were measured using a light microscope and digital camera as a semi-quantitative method. Total duration of spermatozoids motility decreased with increase of Mn concentration in all treatments. This study indicated that Mn could seriously affect the reproductive success of the Caspian lamprey in a polluted environment through decreasing the duration of spermatozoid motility and possibly fertilization and hatching rates. Hence, contact to this metal could decrease the survival rate of this endangered species, especially during the reproduction.
Keywords
Fish
Motility indices
pollution
reproduction
Abascal, F.J., Cosson, J. and Fauvel, C. Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility. J. Fish Biol., 2007; 7: 509-522.
Abdullah, S., Javed, M. and Javid, A. Studies on Acute Toxicity of Metals to the Fish (Labeo rohita). Int. J. Agr. Biol., 2007; 9: 333-337.
Alavi, S.M.H., Cosson, J., Karami, M., Mojazi Amiri, B. and Akhoundzadeh, M. Spermatozoa motility in the Persian Sturgeon, Acipenser persicus: Effect of pH, dilution rate, ions and osmolality. Reprod. Res., 2004; 128: 819-828.
Baynes, S.M., Scott, A.P. and Dawson, A.P. Rainbow trout Salmo gairdneri Richardson, spermatozoa: effects of cations and pH on motility. J. Fish Biol., 1981; 19: 259-267.
Billard, R. and Cosson, M.P. Some problems related to the assessment of sperm motility in freshwater fish. J. Exp. Zool., 1992; 26: 122-131.
Ciereszko, A., Glogowski, J. and Dabrowski, K. Fertilization in landlocked sea lamprey: storage of gametes, optimal sperm: egg ratio, and methods of assessing fertilization success. Fish Biol., 2000; 56: 495-505.
Close, D.A., Fitzpatrick, M.S. and Li, H.W. The ecological and cultural importance of a species of extinction, Pacific lamprey. Fisheries, 2002; 27: 19-25.
Coad, B.W. Fresh water fishes of Iran. Retrieved from http:// www. briancoad.com/ contents.htm. On: 19 May 2014.
Cosson, J., Billard, R., Gibert, C., Dreanno, C. and Suquet, M. The male gamete: from basic to clinical applications. In: Gagnon C (Ed.), Ionic factors regulating the motility of fish sperm. In the male gamete. From basic to clinical application. Cache Rive Press. 1999; 161-185.
Dietrich, G.J., Dietrich, M., Kowalski, R.K., Dobosz, S., Karol, H., Demianowicz, W. and Glogowski, J. Exposure of rainbow trout milt to mercury and cadmium alters sperm motility parameters and reproductive success. Aquat. Toxicol., 2010; 97(4): 277-284.
Fadakar Masouleh, F., Mojazi Amiri, B., Mirvaghefi, A.R. and Nemtollahi, M.A. In vitro Effects of Diazinon on Male Reproductive Tissue and Sperm Motility of Caspian Kutum (Rutilus frisii kutum). J. Environ. Tox., 2011; 5: 108-116.
Fauvel, C., Suquet, M. and Cosson, J. Evaluation of fish sperm quality. J. Appl. Ichthyol., 2010; 26: 636-643.
Jaana, H. and Yamamoto T.S. The ultrastructure of spermatozoa with a note on the formation of the acrosomal filament in the lamprey, Lampetra japonica. Jpn. J. Ichthyol., 1981; 28: 135-141.
Kandarakis, E.D., Bourguignon, G.P., Giudice, L.C., Hauser, R., Prins, G.S., Soto, A.M., Zoeller, R.T. and Gore, A.C. Endocrine-Disrupting Chemicals: An Endocrine Society Scientific Statement. Endocr Rev., 2009; 30(4): 293-342.
Kharat-Sadeghi, M. and Karbasi, A.A.R. The concentration and sources of heavy metals in the sediments of Shirud river. J. Environ. Sci. Technol., 2006; 3(30): 42-52.
Kime, D.E., Ebrahimi, M., Nysten, K., Roelants, I., Rurangwa, E., Moore, H.D.M. and Ollevier, F. Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish .Application to the effects of heavy metals. Aquat. Toxicol., 1996; 36: 223-237.
Kobayashi, W. Effect of osmolality on the motility of sperm from lamprey, Lampetra japonica. Zool. Sci., 1993; 10: 281-285.
Liley, N.R., Tamkee, P., Tsai, R. and Hoysak, D.J. Fertilization dynamics in rainbow trout (Oncorhynchus mykiss): effect of male age, social experience, and sperm concentration and motility on in vitro fertilization. Can. J. Fish. Aquat. Sci., 2002; 52: 144-152.
Linhart, O., Mims, S.D. and Shelton, W.L. Motility of spermatozoa from shovelnose sturgeon (Scaphirhynchus platoryneclus Rafinesque 1820) and paddlefish (Polyodon spathulla Walbaum, 1797). J. Fish Biol., 1995; 97: 902-909.
Satari, M., Shahsavani, D. and Shafiei, S.H. Systematic Icthyology. Hagh Shenas. 2002; 220 P. (In Persian)
Sindayigaya, E.R., Van Cauwenbergh, H. and Robberecht Deelstra, H. Copper, zinc, manganese, iron, lead, cadmium, mercury and rsenic in fish from lake Tanganyika, Burundi. Sci. Total Environ., 1994; 144: 103-115.
ECOPERSIA
Volume 3, Issue 2 - Serial Number 12
June 2015
Pages 1023-1029