Effect of EDTA Application on Lead and Zinc Uptake and Germination of Thlaspi caerulescens L. in a Contaminated Soil

Authors
1 Assistant Professor, Department of Range and Watershed Management, Faculty of Water and Soil, University of Zabol, Iran
2 Former M.Sc. Student of Range Management, Department of Range and Watershed Management, Faculty of Water and Soil, University of Zabol, Iran
Abstract
Pot experiment was carried out to investigate the effects of ethylenediaminetetraacetic acid (EDTA) on some morphological characteristics of Thlaspi caerulescens L., and also on the accumulation of lead (Pb) and zinc (Zn) in roots and shoots of T. caerulescens L.. Experiments were then set up in three treated pots with doses of 3, 6 and 9 mmol kg-1 of EDTA and control pots (C: uncontaminated soil and W: contaminated soil). The results indicated the significant effect of EDTA on morphological characteristics and accumulation of heavy metals in the plant (P<0.05). Data revealed that the maximum of germination (99.11 and 96.00%), maximum of root length (73.31 and 70.14 mm) and maximum of shoot length (51.64 and 44.14 mm) and maximum of biomass weight (61.31 and 52.18 mg) were achieved by C treatment followed by W treatment. The maximum bioconcentration factor (3.57) and translocation factor (0.89) was observed on 9 mmol kg-1 EDTA. In addition, the effect of EDTA on Tolerance Index (TI) showed that the TI decreased with increasing doses of EDTA. The findings indicated that the study species tolerated heavy metals concentration. EDTA had potential to promote the uptake of heavy metals for T. caerulescens L., butwith respect to non-significant differences between 6 mmol kg-1 EDTA and 9 mmol kg-1 EDTA treatments. Thereore, low dose of EDTA suggested to be applied because of its environmental risk.
Keywords

Ait Ali, N., Pilar Bernal, M. and Mohammed, A. Tolerance and bioaccumulation of cadmium by Phragmites australis grown in the presence of elevated concentrations of cadmium, copper, and zinc. Aquat. Bot.,2004; 80: 163-176.
Alpine Pennycress. Nature Gate. Retrieved, 2013; 12-24.
Antosiewicz, D.M., Escude-Duran, C., Wierzbowska, E. and Sklodowska, A.A. Indigenous plant species with the potential for the phytoremediation of arsenic and metals contaminated soil. Water, Air, Soil Pollut., 2008; 193: 197-210.
Baker, A.J.M. Accumulators and excluders strategies in the response of plants to heavy metals. J. Plant Nutr., 1981; 31: 643-654.
Bareen, F. and Tahira, S.A. Efficiency of seven different cultivated plant species for phytoextraction of toxic metals from Tannery effluent contaminated soil using EDTA. Soil Sediment Contam. J., 2010; 19: 160-173.
Berry, J.W., Chappell, D.G. and Barnes, R.B. Improved method of flame photometry. Ind. Eng. Chem. Anal. Edit.,1946; 18: 19-24.
Bisone, S., Blais, J.F. and Mercier, G. Counter-current metal leaching and precipitation for soil remediation. Soil Sediment Contam., 2014; 22: 856-875.
Black, C.A, Evans, D.D, White, J.L., Ensminger, L.E. and Clark, F.E. Methods of Soil Analysis, ASA, Madison, Wisconsin. 1965; 770 P.
Bower, C.A. and Hatcher, J.T. Simultaneous determination of surface area and cation exchange capacity. Soil Sci. Soc. Am. Proc., 1966; 30: 525-527.
Chen, L., Luo, S., Li, X., Wan, Y., Chen, J. and Liu, C. Interaction of Cd-hyperaccumulator Solanum nigrum L. and functional endophyte Pseudomonas sp. Lk9 on soil heavy metals uptake. Soil Biol. Biochem, 2014; 68: 300-308.
Chen, Y., Li, X. and Shen, Z. Leaching and uptake of heavy metals by ten different species of plants during an EDTA- assisted phytoextraction process. Chemosphere, 2004; 57: 187-196.
Du Laing, G., Tack, F.M.G. and Verloo, M.G. Performance of selected destruction methods for the determination of heavy metals in reed plants (Phragmites australis). Anal. Chim. Acta., 2003; 497: 191-198.
Ebrahimi, M. Effect of EDTA treatment method on leaching of Pb and Cr by Phragmites australis (Cav.) Trin. Ex Steudel (common reed). Caspian J. Env. Sci., 2015; 13: 153-166.
Ebrahimi, M. Effect of EDTA and DTPA on phytoremediation of Pb-Zn contaminated soils by Eucalyptus camaldulensis Dehnh and Effect on Treatment Time. Desert, 2014a; 19: 65-73.
Ebrahimi, M. The Effect of EDTA addition on the phytoremediation efficiency of Pb and Cr by Echinochloa crus galii (L.) Beave and associated potential leaching risk. Soil Sediment. Contam., 2014b; 23: 245-256.
Ebrahimi, M., Jafari, M., and Tavili, A. Improved phytoextraction capacity of Prosopis Cineraria (L.) Durce grown on contaminated soil: Roles of EDTA and DTPA treatment time. J. Mater. Environ. Sci., 2015; 6: 1646-1653.
Evangelou, M.W.H., Ebel, M. and Schaeffer, A. Chelate assisted phytoextraction of heavy metals from soil effect, mechanism, toxicity, and fate of chelating agents. Chemosphere, 2007; 68: 989-1003.
Evangelou, M.W.H., Ebel, M. and Schaeffer, A. Evaluation of the effect of small organic acids on phytoextraction of Cu and Pb from soil with tobacco (Nicotiana tabacum). Chemosphere, 2006; 63: 996-1004.
Ghaderian, S.M. and Nosouhi, S. The capability of uptake and removal of toxic heavy metals from the industrial discharge of Mobarakeh Steel Complex by some metal accumulating plants. J. Environ. Studies, 2014; 40: 153-162. (In Persian)
Grčman, H., Vodnic, D., Velikonja-Bolta, S. and Leštan, D. Ethylenediamine disuccinate as a new chelate for environmentally safe enhanced lead phytoremediation. J. Environ. Qual., 2003; 32: 500-506.
Hong, P.K.A and Jiang, W. Factors in the selection of chelating agents for extraction of lead from contaminated soil: effectiveness, selectivity and recoverability. Nowack, B. and Van Briesen, J. (Eds.). Biogeochemistry of Chelating Agents, ACS Symposium Series, Am. Chem. Soc., 2005; 421-431.
Ingrouille, M.J. and Smirnoff, N. Thlaspi Caerulescens J. and C. presl. (T. alpestre L.) in Britain, New Phytol, 1986; 102: 219-233.
Jarvis, M.D. and Leung, D.W.M. Chelated lead transport in Pinus radiata: an ultrastructural study.J. Environ. Exp. Bot., 2002; 48: 21-32.
Johnson, C.E. and Petras, R.J. Distribution of zinc and lead fractions within a forest Spodosol. Soil Sci. Soc. Am. J., 1998; 62: 782-789.
Kos, B. and Leštan, D. Induced phytoextraction/soil washing of lead using biodegradable chelate and permeable barriersSearch Results. Environ. Sci. Technol., 2003; 37: 624-629.
Lombi, E., Zhao, F.J., Dunham, S.J. and McGrath, S.P. Phytoremediation of heavy-metal contaminated soils: natural hyperaccumulation versus chemically enhanced phytoextraction. J. Environ. Qual., 2001; 30: 1919-1926.
Maguire, J.D. Speed of germination: Aid in selection and evaluation of seedling emergence and vigor. Jpn. J. Crop Sci., 1962; 2: 176-177.
Mattina, M.J., Lannucci-Berger, W., Musante, C. and White, J.C. Concurrent plant uptake of heavy metals and persistent organic pollutants from soil. Environ. Pollut., 2003; 124: 375-378.
Meers, E., Ruttens, A., Hopgood, M.J., Samson, D. and Tack, F.M.G. Comparison of EDTA and EDDS as potential soil amendments for enhanced phytoextraction of heavy metals. Chemosphere, 2005; 58: 1011-1022.
Morel, J.L., Mench, M. and Guckert, A. Measurement of Pb2+, Cu2+ and Cd2+ binding with mucilage exudates from Maize (Zea mays L.) roots. J. Biol. Fert. Soils, 1986; 2: 29-34.
Olsen, S.R and Sommers, L.E. Methods of Soil Analysis: Phosphorus. Chemical and microbiological properties, Page, A.L., Miller, R.H., Keeney, D.R (Eds.), Soil Science Society of America, Madison, Wisconsin. 1982; 403-430.
Quiroz, A., Garcia, F.E. and Ilangovan, K. Effects of natural hydrosoluble chelates of three plant species on the mobilization of heavy metals. B. Environ. Contam. Toxicol., 2002; 68: 862-869.
Reeves, R.D. and Baker, A.J.M. Metal-accumulating plants. Phytoremediation of Toxic Metals Using Plants to Clean up the Environment, Wiley Press, New York, USA. 2000; 304 P.
Reeves, R.D., Macfarlane, R.M. and Brooks, R.R. Accumulation of nickel and zinc by western north American genera containing serpentine-tolerant species. Am. J. Bot., 1983; 70: 1297-1303.
Rhoades, J.D. Salinity: Electrical conductivity and total dissolved solids, Methods of Soil Analysis, Page, A. L. (Ed.), American Society of Agronomy, Madison, Wisconsin, 1996; 417-435.
Ruley, A.T., Sharma, N.C., Sahi, S.V., Singh S.R. and Sajwan, K.S. Effects of lead and chelators on growth, photosynthetic activity and Pb uptake in Sesbania drummondii grown in soil. Environ. Pollut., 2006; 144: 11-18.
Saifullah. Meers, E., Qadir, M., Caritat, P., Tack, F.M.G., Du Laing, G. and Zia, M.H. EDTA assisted Pb phytoextraction. Chemosphere,2009; 74: 1279-1291.
Salt, D.E., Pickering, I.J., Prince, R.C., Gleba, D., Dushenkov, S., Smith, R.D. and Raskin, I. Metal accumulation by aquacultured seedlings of Indian mustard. Environ. Sci. Technol., 1997; 31: 1636-1644.
Shahid, M., Austruy, A., Echevarria, G., Arshad, M., Sansullah, M., Aslam, M., Nadeem, M., Nasim, W. and Dumat, C. EDTA-enhanced phytoremediation of heavy metals: A review. Soil Sediment Contam., 2014; 23: 389-416.
Thomas, G.W. Methods of soil analysis: Soil pH and soil acidity, Sparks, D.L. (Ed.). Soil Science Society of America, Madison, Wisconsin, 1996; 475-490.
Turgut, C., Katie, M.P. and Teresa, J.C. The effect of EDTA on Helianthus annuus uptake, selectivity, and translocation of heavy metals when grown in Ohio, New Mexico and Colombia soils. Chemosphere, 2005; 58: 1087-1095.
Usman, A.R.A. and Mohamed, H.M. Effect of microbial inoculation and EDTA on the uptake and translocation of heavy metal by corn and sunflower. Chemosphere, 2009; 76: 893-899.
Vassil, A.D., Kapulnik, Y., Raskin, I. and Salt, D.E. The role of EDTA in lead transport and accumulation by Indian mustard. Plant Physiol., 1998; 117: 447-453.
Wang, A., Luo, C., Yang, R., Chen, Y., Shen, Z. and Li, X. Metal leaching along soil profiles after the EDDS application–A field study. Environ. Pollut., 2012; 164: 204-210.
Wang, X., Ying, W., Qaisar, M., Ejazul, I., Xiaofen, J., Tingqiang, L., Xiaoe, Y. and Dan, L. The effect of EDDS addition on the phytoextraction efficiency from Pb contaminated soil by Sedum alfredii Hance. J. Hazard Mater, 2009; 168: 530-535.
Wenzel, W.W., Unterbrunner, R., Sommer, P. and Sacco, P. Chelate-assisted phytoextraction using canola (Brassica napus L.) in outdoors pot and lysimeter experiments. Plant Soil, 2003; 249: 83-96.
Yang, J.Y., Yang, X.E., He, Z.L., Li, T.Q., Shentu, J.L. and Stoffella, P.J. Effects of pH, organic aciads, and inorganic ions on lead desorption from soils. Environ. Pollut. 2006; 143: 9-15.
Yoon, J., Cao, X., Zhou, Q. and Ma, L.Q. Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Sci. Total Environ., 2006; 368: 456-464.
Zaier, H., Ghnaya, T., Ben Rejeb, K., Lakhdar, A., Rejeb, S. and Jemal, F. Effects of EDTA on phytoextraction of heavy metals (Zn, Mn and Pb) from sludge-amended soil with Brassica napus, Bioresource Technol., 2010; 101: 3978-3983.
Zhao, F.J. and McGrath, S.P. Biofortification and phytoremediation. Current Opinion. Plant Biol., 2009; 12: 373-380.
Zhao, H.Y., Lin, L.J., Yan, Q.L., Yang, Y.X., Zhu, X.M. and Shao, J.R. Effects of EDTA and DTPA on Lead and Zinc Accumulation of Ryegrass. J. Environ. Prot., 2011; 2: 932-939.